Query: ISSN: "0031-5850"
|Authors||D.G. Knapp, G.M. Kovács, E. Zajta, J.Z. Groenwald, P.W. Crous|
|Title||Dark septate endophytic pleosporalean genera from semiarid areas|
|Journal||Persoonia - Molecular Phylogeny and Evolution of Fungi|
|Keywords||Dothideomycetes; endophytes; Massarineae; mating; phylogeny; sexual state; taxonomy|
|Abstract||Dark septate endophytes (DSE) are distributed worldwide as root-colonising fungi, and frequent in environments with strong abiotic stress. DSE is not a taxon, but constitutes numerous fungal taxa belonging to several orders of Ascomycota. In this study we investigate three unidentiﬁed DSE lineages belonging to Pleosporales that were found previously in semiarid sandy grasslands. For molecular phylogenetic studies seven loci (ITS, partial 18S nrRNA, 28S nrRNA, actin, calmodulin, transcription-elongation factor 1-α and ß-tubulin genes) were ampliﬁed and sequenced. Based on morphology and the resulting molecular phylogeny these isolates were found to represent three novel genera within the Pleosporales, namely Aquilomyces, Flavomyces and Darksidea, with eight novel species. Molecular data revealed that monotypic Aquilomyces belongs to Morosphaeriaceae, monotypic Flavomyces represents an incertae sedis lineage related to Massarinaceae, and Darksidea, with six new species, is allied to the Lentitheciaceae. During this study we tested numerous conditions to induce sporulation, and managed for the ﬁrst time to induce several DSE to form their sexual morphs.|
|Download paper|| http://www.repository.naturalis.nl/document/622096 |