Query: keyword: "ITS"
|Authors||I. Olariaga, N. van Vooren, M. Carbone, K. Hansen|
|Title||A monograph of Otidea (Pyronemataceae, Pezizomycetes)|
|Journal||Persoonia - Molecular Phylogeny and Evolution of Fungi|
|Keywords||Flavoscypha; ITS; ITS1 minisatellites; LSU; Otideopsis; resinous exudates|
|Abstract||The easily recognised genus Otidea is subjected to numerous problems in species identiﬁcation. A number of old names have undergone various interpretations, materials from different continents have not been compared and misidentiﬁcations occur commonly. In this context, Otidea is monographed, based on our multiple gene phylogenies assessing species boundaries and comparative morphological characters (see Hansen & Olariaga 2015). All names combined in or synonymised with Otidea are dealt with. Thirty-three species are treated, with full descriptions and colour illustrations provided for 25 of these. Five new species are described, viz. O. borealis, O. brunneoparva, O. oregonensis, O. pseudoleporina and O. subformicarum. Otidea cantharella var. minor and O. onotica var. brevispora are elevated to species rank. Otideopsis kaushalii is combined in the genus Otidea. A key to the species of Otidea is given. An LSU dataset containing 167 sequences (with 44 newly generated in this study) is analysed to place collections and determine whether the named Otidea sequences in GenBank were identiﬁed correctly. Fourty-nine new ITS sequences were generated in this study. The ITS region is too variable to align across Otidea, but had low intraspeciﬁc variation and it aided in species identiﬁcations. Thirty type collections were studied, and ITS and LSU sequences are provided for 12 of these. A neotype is designated for O. cantharella and epitypes for O. concinna, O. leporina and O. onotica, along with several lectotypiﬁcations. The apothecial colour and shape, and spore characters are important for species identiﬁcation. We conclude that to distinguish closely related or morphologically similar species, a combination of additional features are needed, i.e. the shape of the paraphyses, ectal excipulum structure, types of ectal excipulum resinous exudates and their reactions in Melzer’s reagent and KOH, tomentum and basal mycelium colours and exudates. The KOH reaction of excipular resinous exudates and basal mycelium are introduced as novel taxonomic characters.|
|Download paper|| http://www.repository.naturalis.nl/document/622100 |