Amanita grallipes, a new species from the State of Parand in southern Brazil, belonging to Amanita sect. Lepidella subsect. Vittadiniae, is extensively described and illustrated. It is well-characterized by a long, slightly rooting stipe with a non-bulbous base and scattered remnants of the universal veil, a dark brown pileus with dark brown warts, lamellae turning maize yellow, ellipsoid spores shorter than 9.5 µm and the presence of clamp-connections.