An enumeration is presented of species of macrofungi considered to be threatened in the Netherlands. The list comprises 944 species, including 91 (presumably) extinct species and 182 species which are directly threatened with extinction. In addition for each species information is given on preferential habitat, substrate, if possible the associated organisms, way of habitat exploitation, estimated frequency before 1950 and after 1970 and presence in Red Data Lists of other European countries. The criteria used for placing a species on the Red Data List are briefly discussed. Some statistical data are presented on the proportional contribution of various taxonomic and ecological groups to the list. The most important causes for the decrease of threatened macrofungi are concisely discussed and some measures for conservation of macrofungi are recommended.