For the first time a systematic study of the types and authentic specimens of agarics and boletes preserved in the Persoon Herbarium has been carried out. Some aspects of the possibilities of type analysis with regard to specimens over 130 year old are discussed. 137 species were analyzed. The following new combinations are proposed: Mycena amygdalina (Pers.) Sing., Psilocybe angulata (Batsch ex Pers.) Sing., Inocybe argillacea (Pers. ex Pers.) Sing., Lepiota aspera var. acutesquamosa (Weinm.) Sing., Pseudoclitocybe bacillaris (Pers.) Sing., Acurtis chalybeus (Pers. ex Fr.) Sing., Hemimycena cucullata (Pers. ex Fr.) Sing., Tubaria dispersa (Pers.) Sing., Omphalia grossula (Pers.) Sing., Campanella merulina (Pers.) Sing., Mycena phyllogena (Pers.) Sing., Galerina laevis (Pers.) Sing., Galerina pumila (Pers. ex Fr.) Sing., Resupinatus tricholis (Pers.) Sing. A new name, Collybia kuehneriana Sing., is proposed.